Original Research Article
Year: 2019 | Month: April-March | Volume: 4 | Issue: 2 | Pages: 1-10
Skeletal Effect of Lamotrigine Administration in Pregnant Albino Rats
Muna Anwer Mohammed Kutb1, Huda Adulwadood Mohammed Omer2
1Department of Morphology, Histopathology Unit, Faculty of Medicine, Aden University, Yemen, Aden- AL- Maalla. Yemen.
2Department of Paraclinic, Forensic Medicine & Toxicology Unit, Faculty of Medicine, Aden University, Yemen, Aden- AL-Mansorrha. Yemen
Corresponding Author: Muna Anwer Mohammed Kutb
ABSTRACT
Background: Lamotrigine is a broad-spectrum agent that was approved for use in 1994 as an adjunctive treatment in adults with partial-onset seizures, later approval was granted for use in adults and children aged 2 years and older with generalized seizures associated with Lennox – Gastaut syndrome (childhood epileptic encephalopathy) and for conversion to monotherapy.
Methods: Sixty adult non pregnant female albino rats and 30 male rats of the same strain weighted (150-200 grams) purchased from animal house of faculty of medicine, Assiut University. Animals were classified randomly into three groups; each group contained 20 pregnant female rats. Control groups(I), B-Treated groups: IIa: received therapeutic dose of lamotrigine (5.4 mg/day) and IIb: received ¼ LD50 of lamotrigine (32 mg/kg). The fetuses in this subgroup were treated according to modified Dawson’s technique for staining fetal skeleton. The skeletal system examination included: I) Bones of axial skeleton and II) Bones of the appendicular skeleton.
Result: There is a very highly statistical significant reduction in the mean of complete craniofacial ossification centers of treated groups in comparison with the control group. Also reduction in the mean of complete vertebral, thoracic, lumber, sacral, caudal, matacarbal and metatarsal ossification centers of IIb group.
Conclusion: Lamotrigine in therapeutic dose is the drug of choice to be used by epileptic pregnant women.
Keywords: Lamotrigine, skeletal examination, therapeutic dose, albino rat, ¼ LD50, ossification centers.