Original Research Article
Year: 2022 | Month: January-March | Volume: 7 | Issue: 1 | Pages: 54-64
DOI: https://doi.org/10.52403/ijshr.20220111
Cellular Changes and Effect on DNA Integrity of the Epididymal Cells of Swiss Albino Mice Post Exposure to High Dose of Cyclophosphamide: A Histological and TUNEL Assay Study
Supriya1, Sneha G Kalthur2, Guruprasad Kalthur3, Guruprasad Nayak4, Sandhya Kumari5
1Assistant Professor, Anatomy Department, Rajarajeswari Medical College and Hospital, Bengaluru- 560074, Karnataka.
2Professor, Anatomy Department, Kasturba Medical College, Manipal- 576104, Karnataka.
3Professor, 4,5Postgraduates, Department of Clinical Embryology, Kasturba Hospital, Manipal-576 104, Karnataka.
Corresponding Author: Supriya
ABSTRACT
Background: Many studies done in past literature focuses on gonadal toxicity of Cyclophosphamide, but very less studies are done on the epididymis post exposure to this drug. Hence a sincere attempt is made in present study to look into the cellular changes in the epididymis through histology and TUNEL Assay.
Subjects & Methods: Healthy male Swiss albino adult mice, were obtained from Central Animal Research Facility, Manipal were used for the study.
The adult as well as prepubertal male mice were divided into control and test groups. In test group, the mice were injected with Cyclophosphamide at variable doses. From each group certain no. of mice was sacrificed on different days. A control group was kept for each intervals. The epididymal tissue was then extracted and used in histological and Tunnel Assay study.
Results: Even though there was no significant change in the number of basal cells, the apical cells decreased marginally at higher doses of CP. A TUNEL assay was performed in the epididymal sections to understand its effect on the DNA integrity. Up to 100 mg/kg, CP did not induce any significant increase in the TUNEL positive tubules. However, at 200 mg/kg dose and above, there was an increase in the number of tubules with TUNEL positive cells indicating its DNA damaging effect. However, this increase was statistically not significant
Conclusion: The present study highlights the need for looking into the cellular damage of the cells after exposure to one of the commonly used anticancer drug- cyclophosphamide.
Keywords: Epididymal Histology, Tunnel Assay, Cyclophosphamide, Swiss Albino mice.