Original Research Article
Year: 2020 | Month: April-June | Volume: 5 | Issue: 2 | Pages: 81-87
Phytochemical and Antibacterial Activities of Anacardium occidentale fruits extracts (Cashew) on two Drug Resistant Bacteria
Okey-Ndeche Florence Ngozika 1, Unegbu Valentine Nnachetam2, Obum-Nnadi Charity Ndidi3, Anyaegbunam Bede Chike4
1,3,4Department of Microbiology, Faculty of Natural and Applied Sciences, Veritas University, Abuja, Nigeria.
2Department of Microbiology, Spiritan University, Nneochi, Abia State, Nigeria.
Corresponding Author: Unegbu Valentine Nnachetam
ABSTRACT
This present study was aimed at investigating the phytochemical and antibacterial activities of both aqueous and ethanolic extracts of Anacardium occidentale (cashew) fruit on Staphylococcus aureus and Escherichia coli. The Phytochemical constituents of this medicinal plant were carried out using standard methods. Agar well diffusion method was used to determine the antibacterial activity of the plant extracts. The Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of the plant extracts on the test isolates were determined by the Broth dilution method. Phytochemical analysis showed that Phenols, alkaloids, anthraquinolones, flavonoids, glycosides, tannins, glycoside, terpenoids and tannins were present in both ethanolic and aqueous extracts of A. occidentale. The antibacterial activities of aqueous and ethanolic fruit extract of Anacardium occidentale showed that the mean zone diameter of inhibition for S. aureus on the different extracts was between the range of 11mm to 28mm while that of E. coli was between 17mm to 29mm. The MIC of different extracts of S. aureus was between 6.25mg/ml to 25mg/ml while that of E. coli was also between 6.25mg/ml to 25mg/ml. The MBC of different extracts of S. aureus isolates were between the ranges of 100 to 150mg/ml while that of E. coli was also between the range of 100 to 150mg/ml. The ethanol and aqueous extracts of Anacardium occidentale (Cashew) fruits are rich in wide range of secondary metabolites. Both extracts exhibited bactericidal activities against S. aureus and E. coli. The antibacterial efficacy of the fruit extracts of A. occidentale lends credence to ethno-medicinal use of the plant to treat various ailments.
Keywords: Anacardium occidentale, Ethanolic, Aqueous, Staphylococcus aureus, Escherichia coli