Original Research Article
Year: 2019 | Month: October-December | Volume: 4 | Issue: 4 | Pages: 131-141
Estimation of Time since Death by Histological Examination of Proximal Convoluted Tubule in Human Kidney
Rajni Thakur1, Anjana Tiwari2
1Assistant Professor, Department of Anatomy, Pt.J.N.M.Medical College, Raipur, (C.G.),
2Associate Professor, Department of P.S.M, Chandulal Chandrakar Medical College, Durg, (C.G.)
Corresponding Author: Rajni Thakur
ABSTRACT
Background: Proximal tubules are highly convoluted in the cortex and become straighter toward the medulla. Proximal tubules are round to oval. Usually round nuclei are in the center or toward the base of each cell. Their wall made of simple cuboidal or low columnar epithelium; surround a central, irregularly shaped lumen. It is important for the histopathologist to differentiate postmortem autolysis from ante mortem coagulative necrosis. Histologically cells react similarly to both forms of self-digestion.
Aim: Estimation of time since death by histological examination of proximal convoluted tubules.
Materials and Methods: In this research we obtained 40 Human kidneys samples at different time intervals between 4hrs to 52.30 hr, temperature between17.3/22.3-31.3/450C, humidity between 11/36 to 75/95, different age and sex from cadavers with a known time of death where death had resulted from natural death, suffocation and trauma, dead individual health and not suffering from disease affecting kidney and excluded all kidney disease cadaver.
Result: postmortem histological changes were seen in PCT i.e. retraction as well as disruption (fragmented) of epithelium from the basement membrane although the cells were with nuclei after 4hrs (27.5/42.20C, T).
Conclusion: In Proximal convoluted tubules lumens were closed due to swelling of epithelial cells. Retraction of epithelium from the basement membrane and its disruption with individualization of cells, nuclear pyknosis, karyolysis and loss of tubular architecture with debris in the lumen were observed in Proximal convoluted tubules, This research could be used to estimation of the time after death.
Keywords: Proximal convoluted tubules, Vesicular nuclei, Ancient period, edematous, disruption.